WASHINGTON — White House press officials altered the official transcript of a call in which President Biden appeared to take a swipe at supporters of President Trump, drawing objections from the federal employees who document such remarks for posterity.

Mr. Biden created an uproar earlier this week with his remarks to Latino activists responding to the comedian Tony Hinchcliffe, who at Trump’s Madison Square Garden rally referred to Puerto Rico as a “floating island of garbage.”

Mr. Biden, according to a transcript prepared by the official White House stenographers, told the Latino group on a Tuesday evening video call, “The only garbage I see floating out there is his supporters — his — his demonization of Latinos is unconscionable, and it’s un-American.”

The transcript released by the White House press office, however, rendered the quote with an apostrophe, reading “supporter’s” rather than “supporters,” which aides said pointed to Mr. Biden criticizing Mr. Hinchcliffe, not the millions of Americans who are supporting Trump for president.

The change was made after the press office “conferred with the president,” according to an internal email from the head of the stenographers’ office. 

The authenticity of the email was confirmed by two government officials who spoke on condition of anonymity to discuss internal matters.

The supervisor, in the email, called the press office’s handling of the matter “a breach of protocol and spoliation of transcript integrity between the Stenography and Press Offices.”

“If there is a difference in interpretation, the Press Office may choose to withhold the transcript but cannot edit it independently,” the supervisor wrote, adding, “Our Stenography Office transcript — released to our distro, which includes the National Archives — is now different than the version edited and released to the public by Press Office staff.”

The edit of the transcript came as the White House scrambled to respond to a wave of queries from reporters about Mr. Biden’s comments. 

The president’s remarks clashed with Vice President Harris’s near-simultaneous speech outside the White House in which she called for treating Americans of differing ideologies with respect.

The Trump campaign quickly moved to fundraise off the quote, and the next day, Trump himself held a photo op inside a garbage truck to try to capitalize on Mr. Biden’s criticism.

Ms. Harris on Wednesday distanced herself from Mr. Biden’s comments — making the clearest break from the president since she took over for him at the top of the Democratic ticket just over three months ago. “Let me be clear,” she told reporters, “I strongly disagree with any criticism of people based on who they vote for.”

According to the email, the press office had asked the stenographers to quickly produce a transcript of the call amid the firestorm. 

Mr. Biden himself took to social media to say that he was not calling all Trump supporters garbage and that he was referring specifically to the “hateful rhetoric about Puerto Rico spewed by Trump’s supporter at his Madison Square Garden rally.”

The stenographers office is charged with preparing accurate transcripts of public and private remarks of the president for preservation by the National Archives and distribution to the public.

The two-person stenography team on duty that evening — a “typer” and “proofer” — said any edit to the transcript would have to be approved by their supervisor, the head of stenographers’ office.

The supervisor was not immediately available to review the audio, but the press office went ahead and published the altered transcript on the White House website and distributed it to press and on social media in an effort to tamp down the story.

The White House senior deputy press secretary, Andrew Bates, that evening also posted on X the edited version of the quote and wrote that Mr. Biden was referring ”to the hateful rhetoric at the Madison Square Garden rally as ‘garbage.’”

The supervisor, a career employee of the White House, raised the concerns about the press office action — but did not weigh in on the accuracy of the edit — in an email to the White House communications director, Ben LaBolt, press secretary Karine Jean-Pierre, and other press and communications officials.

“Regardless of urgency, it is essential to our transcripts’ authenticity and legitimacy that we adhere to consistent protocol for requesting edits, approval, and release,” the supervisor wrote.

The supervisor declined to comment to the AP and referred questions about the matter to the White House press office.

Asked to comment, Mr. Bates did not address the alteration of the transcript and said: “The President confirmed in his tweet on Tuesday evening that he was addressing the hateful rhetoric from the comedian at Trump’s Madison Square Garden rally. That was reflected in the transcript.”

House Republicans, meanwhile, were debating launching an investigation into the matter. The House Republican conference chairwoman, Congresswoman Elise Stefanik, and the House oversight committee chairman, Congressman James Comer, on Wednesday accused White House staff of “releasing a false transcript” of Mr. Biden’s remarks.

In a letter to White House counsel Ed Siskel on Wednesday, they called on the administration to retain documents and internal communications related to Biden’s remarks and the release of the transcript.

“White House staff cannot rewrite the words of the President of the United States to be more politically on message,” the lawmakers wrote to Mr. Siskel.

Ms. Stefanik and Mr. Comer said the action could be in violation of the Presidential Records Act of 1978.