WASHINGTON – Scientists trying to sniff out biological differences between gay and straight men have found new evidence – in scent.

It turns out that sniffing a chemical from testosterone, the male sex hormone, causes a response in the sexual area of gay men’s brains, just as it does in the brains of straight women, but not in the brains of straight men.

“It is one more piece of evidence … that is showing that sexual orientation is not all learned,” an expert on brain anatomy and sexual orientation at the Michael G. DeGroote School of Medicine at McMaster University in Ontario, Canada, Sandra Witelson, said.

Ms. Witelson, who was not part of the research team that conducted the study, said the findings show a biological involvement in sexual orientation.

The study, published in today’s issue of Proceedings of the National Academy of Sciences, was done by researchers at the Karolinska Institute in Stockholm, Sweden.

They exposed heterosexual men and women and homosexual men to chemicals derived from male and female sex hormones.

These chemicals are thought to be pheromones – molecules known to trigger responses such as defense and sex in many animals.

Whether humans respond to pheromones has been debated, although in 2000 American researchers reported finding a gene that they believe directs a human pheromone receptor in the nose.

The Swedish study was one of a series looking at whether parts of the brain involved in reproduction differ in response to odors and pheromones, lead researcher Ivanka Savic said.

The brains of different groups responded similarly to ordinary odors such as lavender, but differed in their response to the chemicals thought to be pheromones, Ms. Savic said.

The Swedish researchers divided 36 subjects into three groups – heterosexual men, heterosexual women, and homosexual men. They studied the brain response to sniffing the chemicals, using PET scans. All the subjects were healthy, unmedicated, right-handed, and HIV negative.

When they sniffed smells like cedar or lavender, all of the subjects’ brains reacted only in the olfactory region that handles smells.

But when confronted by a chemical from testosterone, portions of the brain that are active in sexual activity were activated in straight women and in gay men, but not in straight men, the researchers found.

The response in gay men and straight women was concentrated in the hypothalamus with a maximum in the preoptic area that is active in hormonal and sensory responses necessary for sexual behavior, the researchers said.

And when estrogen, the female hormone, was used, there was only a response in the olfactory portion of the brains of straight women. Homosexual men had their primary response also in the olfactory area, with a very small reaction in the hypothalamus, while heterosexual men responded strongly in the reproductive region of the brain.

Ms. Savic said the group is also doing a study involving homosexual women, but those results are not yet complete.

In a separate study that looks at people’s response to the body odors of others, researchers in Philadelphia found sharp differences between gay and straight men and women.

“Our findings support the contention that gender preference has a biological component that is reflected in both the production of different body odors and in the perception of and response to body odors,” said neuroscientist Charles Wysocki, who led the study.

In particular, he said, finding differences in body odors between gay and straight individuals indicates a physical difference.

It’s hard to see how a simple choice to be gay or lesbian would influence the production of body odor, he said.

Mr. Wysocki’s team at the Monell Chemical Senses Center studied the response of 82 heterosexual and homosexual men and heterosexual and homosexual women to the odors of underarm sweat collected from 24 donors of varied gender and sexual orientation.